Hi, one my friend AGSer had a trouble when he was creating his game.
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(translation from Russian)
With 32-bit of colour graphics with alpha-channel look normal. But when I use same sprites in GUI or inventory, all alpha channels disappear. You can see it in your game's inventory, althings in there have extra edges.
Curiously, but I won it with imposing GUI's background under another GUI's background with special rules. i.e. turning on of GUI inventory was with showing another GUI under inventory.
But I got good result only in pre-tests. I couldn't fast repeat it.
So who knows, how in the 32-bit game, where sprites look great in the room background, these sprites look same good in the inventory?
The GUI needs to have an alpha-channel background image, or the GUI won't be enabled for alpha blending.
Does the GUI have a Background Image set?